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Image Search Results
Journal: STAR Protocols
Article Title: Protocol to assess the tolerogenic properties of adoptively transferred dendritic cells during murine experimental autoimmune encephalomyelitis
doi: 10.1016/j.xpro.2022.101653
Figure Lengend Snippet:
Article Snippet:
Techniques: Recombinant, Adjuvant, Staining, Software
Journal: Journal of Veterinary Science
Article Title: Immunization of BALB/c mice with Brucella abortus 2308Δ wbkA confers protection against wild-type infection
doi: 10.4142/jvs.2015.16.4.467
Figure Lengend Snippet: Anti- Brucella in serum from mice immunized with 2308Δ wbkA or S19. Mice were inoculated i.p. with 1 × 10 6 CFU of 2308Δ wbkA or S19. Control groups were inoculated with PBS. Serum samples were collected at 3, 7, 14, 21 and 28 days post-vaccination (n = 5 per time point) and IgG antibodies were determined by enzyme-linked immunosorbent assay (ELISA). The results are the means ± SD of the absorbance at 450 nm (OD 450 ). Significant differences between the 2308Δ wbkA and PBS groups are indicated by * p < 0.05.
Article Snippet: The levels of IgG in the supernatants were measured using an
Techniques: Control, Enzyme-linked Immunosorbent Assay
Journal: Journal of Veterinary Science
Article Title: Immunization of BALB/c mice with Brucella abortus 2308Δ wbkA confers protection against wild-type infection
doi: 10.4142/jvs.2015.16.4.467
Figure Lengend Snippet: IFN-γ production by spleen cells of BALB/c mice vaccinated with 2308Δ wbkA or S19. Spleens from mice were inoculated i.p. with 1 × 10 6 CFU of 2308Δ wbkA or S19. Control groups were inoculated with PBS. At 4 weeks post-vaccination, mice were euthanized and splenocytes were recovered and stimulated with either heat-killed S2308, concanavalin A (ConA) or RPMI 1640. Splenocyte culture supernatants were harvested and analysis of IFN-γ secretion (pg/mL) was assayed by ELISA. Significant differences from the same stimulus in PBS-injected mice are indicated by * p < 0.05.
Article Snippet: The levels of IgG in the supernatants were measured using an
Techniques: Control, Enzyme-linked Immunosorbent Assay, Injection
Journal: Journal of Veterinary Science
Article Title: Immunization of BALB/c mice with Brucella abortus 2308Δ wbkA confers protection against wild-type infection
doi: 10.4142/jvs.2015.16.4.467
Figure Lengend Snippet: Humoral immune responses to WbkA were assessed in serum by indirect ELISA. Sera from mice immunized with 2308Δ wbkA , S2308, S19 and PBS were collected. Humoral immune responses against the WbkA protein were not detected in serum from 2308Δ wbkA immunized mice.
Article Snippet: The levels of IgG in the supernatants were measured using an
Techniques: Indirect ELISA